Effect of hypoxia on cerebral cortex differentiation of iPC cells

缺氧对iPC细胞大脑皮层分化的影响

• 批准号: 23618004
• 负责人: TAKASAKI Mami
• 金额: $ 3.49万
• 依托单位: University of Tsukuba
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23618004/
• 关键词: 低酸素応答; 神経; 胚性幹細胞; iPS細胞; 分化; 神経外胚葉; 再生医学; 発生

Embryonic stem (ES) cells are useful for elucidating the molecular mechanisms of cell fate decision in the early development of mammals. It has been shown that aggregate culture of ES cells efficiently induces neuroectoderm differentiation. However, the molecular mechanism that leads to selective neural differentiation in aggregate culture is not fully understood.In this study, we demonstrate that the oxygen-sensitive hypoxia-inducible transcription factor, Hif-1a, is an essential regulator for neural commitment of ES cells. However, we could not observe Hif-1a activation in aggregate culture of human iPS cells at the timing of neural commitment. Further analysis might be necessary to elucidate molecular mechanism of neuroectoderm differentiation for iPS cells.

胚胎干细胞（ES细胞）可用于阐明哺乳动物早期发育中细胞命运决定的分子机制。已经表明，ES细胞的聚集体培养有效地诱导神经外胚层分化。然而，在聚集体culture.In这项研究中，我们证明，氧敏感性缺氧诱导转录因子，HIF-1a，是一个重要的调节神经承诺的ES细胞的分子机制，导致选择性神经分化尚未完全理解。然而，我们不能观察到HIF-1a激活的人iPS细胞的聚集培养在神经承诺的时间。进一步的分析可能是必要的，以阐明神经外胚层分化的iPS细胞的分子机制。

项目成果

The role of hypoxia inducible factor-1a in neural commitment of mouse embryonic stem cells

缺氧诱导因子1a在小鼠胚胎干细胞神经定向中的作用

• 发表时间: 2012
• 作者: Tatsuya Aonuma;Naofumi Takehara;Maki Kabara;Kouki Matsuki;Atsushi Yamauchi;Jun-ichi Kawabe and Naoyuki Hasebe;高崎(松尾)真美
• 通讯作者: 高崎(松尾)真美

Hypoxia inducible factor 1a is positive regulator of mouse embryonic stem cell differentiation into neural progenitors

缺氧诱导因子1a是小鼠胚胎干细胞分化为神经祖细胞的正调节因子

• 发表时间: 2011
• 作者: 山本幸織;近藤大輔;冨田辰之介;宮崎歴;大石勝隆;稲垣博英・豊平由美子・小原剛・蜂須賀徹・柳原延章;高崎(松尾)真美
• 通讯作者: 高崎(松尾)真美