Local translation of nuclear proteins at the synapses, translocation to the nucleus and synaptic plasticity.

我们研究了大鼠Gtf2i基因的启动子区域，并基于cap分析基因表达（CAGE）数据库预测了Gtf2i mRNA的转录起始点。利用这些信息，我们在大鼠脑中鉴定了7个具有不同5' utr的Gtf2i新转录本。我们通过原位杂交检测了这些具有替代5' utr的新型Gtf2i mrna在大鼠脑中的表达。不同5′utr的Gtf2i mRNA表达模式存在差异，部分在神经元细胞的树突加工中检测到，提示具有这些5′utr的Gtf2i mRNA存在于神经元树突/PSD中。我们鉴定了Gtf2i编码序列的四个新的剪接变体，以及两个已知的大鼠脑变体。这些结果表明，Gtf2i mRNA的5' utr在大脑中Gtf2i基因的表达中起着重要作用：通过替代启动子的使用，mRNA在神经元树突中的定位和时空特异性翻译的差异细胞表达。

We investigated promoter region of rat Gtf2i gene and predicted alternative transcription start points of Gtf2i mRNA based on cap analysis gene expression (CAGE) database. Using these information, we identified seven novel transcripts of Gtf2i with different 5'UTRs in rat brain. We examined expression of these novel Gtf2i mRNAs with alternative 5'UTRs in rat brain by in situ hybridization. Gtf2i mRNAs with different 5'UTRs showed differential expression patterns and some of them were detected in dendritic processed of neuronal cells, suggestion that Gtf2i mRNA with these 5'UTRs exist in neuronal dendrite/PSD. We identified four novel splicing variants of the coding sequence of Gtf2i in addition to two known variants in rat brain.These results suggest 5'UTRs of Gtf2i mRNA play important roles in expression of Gtf2i gene in the brain: differential cellular expression via alternative promoter usage, mRNA localization to and spatiotemporally specific translation in neuronal dendrites.