Promotion of the 4Rs in animal experiments by the development of a production process for polyclonal antibodies using a goldfish

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In this study, we tried to the development of a production process for polyclonal antibodies using a particular kind of goldfish, in which lymph can be sustainably and easily collected from its enlarged corneas containing antibodies. We successfully purified polyclonal antibodies in lymph extracted from goldfish and established the detection system for them based on ELISA. Further, we confirmed it by dot blotting that specific antibodies in lymph can be produced by examining adjuvants at the immunization.