Chemical analyses of microflora and feces in lifestyle-related disease animals for the identification of intestinal inflammatory biomarkers

近年来的研究表明，肠道植物群是决定生活方式相关疾病宿主表型的重要因素。此外，由肠道植物群改变引起的低水平但持续的炎症似乎有助于这些严重疾病的发病机制。因此，在本研究中，我们对肠道植物群和粪便进行了化学和统计分析，以确定炎症生物标志物。我们检查了育种条件，样品制备，MS分析，多变量分析等。使用最佳条件，收获的样品进行UPLC-TOF-MS或GC-EI-MS。检测到几个MS离子峰作为炎症生物标志物的候选者。基于这些结果，正在进行进一步的研究以鉴定肠道中的炎症生物标志物。

Recent pioneering studies have revealed that intestinal flora was an important factor to determine the host phenotype associated with lifestyle-related diseases. Moreover low level but continuous inflammation derived from the alteration of intestinal flora appears to contribute to the pathogenesis of these serious diseases. Therefore, in this study, we performed chemical and statistical analysis of intestinal flora and feces to identify the inflammation biomarkers. We examined conditions for the breeding, sample preparations, MS analyses, multivariate analyses, and etc. Using optimum condition, harvested samples were subjected to UPLC-TOF-MS or GC-EI-MS. Several MS ion peaks were detected as a candidate for inflammation biomarker. Based on these results, further study is in progress to identify inflammation biomarker in intestinal tract.