Development of in situ measurement method for growth and metabolic activity of marine archaeal community under euphotic zone in the ocean

海洋真光带下海洋古菌群落生长和代谢活动原位测量方法的建立

• 批准号: 23651004
• 负责人: UTSUMI Motoo
• 金额: $ 2.5万
• 依托单位: University of Tsukuba
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23651004/
• 关键词: 生物海洋; 微生物代謝測定

To know real marine prokaryotic growth rate, I developed in situ incubation machine named ROCS incubation system. This machine collects sea water, and then, incubates marine prokaryotes at the same depth directly with adding some substrate and fixing a certain volume of incubated samples at programed time interval. A field incubation experiment was performed at Suruga Bay, and the ROCS system was worked at 400 m depth for one day long. It was also sampled 400 m depth seawater with a water sampler, and did incubation under near in situ temperature on board. As a result, I have succeeded in establishing in situ incubation system, and the results showed that the growth rates of total bacteria, eubacteria and clenarchaeota under onboard incubation were faster than these growth rates under in situ incubation.

为了了解真实的海洋原核生物生长速度，我研制了原位培养机，命名为ROCS培养系统。该机器收集海水，然后直接在同一深度孵育海洋原核生物，加入一定的底物，按设定的时间间隔固定一定体积的孵育样品。在骏河湾进行了现场孵化实验，并在400 m深度下进行了为期一天的ROCS系统工作。它还用水采样器对400米深的海水进行了采样，并在船上近原地温度下进行了孵育。因此，我成功地建立了原位孵育体系，结果表明，在机载孵育下，总细菌、真细菌和克伦古菌的生长速度要快于原位孵育。

项目成果

In situ incubation for marine prokaryotes using ROCS incubation system.

使用 ROCS 孵化系统对海洋原核生物进行原位孵化。

• 发表时间: 2014
• 作者: Chie Amano;Qintong Li;Seiya Takahashi;Motoo Utsumi.
• 通讯作者: Motoo Utsumi.

In situ measurement of marine bacterial growth rate and community structure using the ROCS incubation system.

使用 ROCS 培养系统原位测量海洋细菌生长速率和群落结构。

• 发表时间: 2012
• 作者: Chie Amano (Sato);Qintong Li;Shohei Akiyama;Masao Uchida;Motoo Utsumi
• 通讯作者: Motoo Utsumi

In situ measurement of marine bacterial growth rate and community structure using the ROCS incubation system

使用 ROCS 培养系统原位测量海洋细菌生长速率和群落结构

• 发表时间: 2012
• 作者: Chie Amano (Sato);Qintong Li;Shohei Akiyama;Masao Uchida;Motoo Utsumi
• 通讯作者: Motoo Utsumi

駿河湾中層の海洋微生物群集増殖速度の測定－ROCS現場培養機による現場水深培養と船上培養との比較－

骏河湾中层海洋微生物群落生长速度的测量 - 使用ROCS原位培养机的原位深水培养与船上培养的比较 -

• 发表时间: 2014
• 作者: 高橋聖弥;天野（佐藤）千恵;李沁潼;内海真生
• 通讯作者: 内海真生

Distribution and structure of planktonic Archaea in the Arctic Ocean using 2008 - 2010 R/V Mirai cruise samples

使用 2008 - 2010 年 R/V Mirai 巡航样本研究北冰洋浮游古菌的分布和结构

• 发表时间: 2011
• 作者: Chie Amano (Sato);Shohei Akiyama;Masao Uchida;Motoo Utsumi
• 通讯作者: Motoo Utsumi