Investigationof mechanisms of radical oxygen species (ROS) via ATP・ATP receptor signaling in the cells exposed to nanoparticles

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The mechanism of ROS production via ATP-ATP signaling was examined in the mouse kidney mesangium cells exposed to nano-silica particles with 30, 70, and 300nm of diameter (nSP30, nSP70, and nSP300, respectively). As a result, the significant ATP release, Ca2+influx and ROS production were observed in the cells exposed to nSP30 and nSP70. These events were blocked by the treatment with ecto-nucleotidase apyrase, suggesting involvement of ATP・ATP receptor signaling in nSP-induced ROS production. Furthermore, the same results were obtained in human keratinocytes, HaCaT cells.