Development of a method for the mechanistic investigation of toxicological endpoints modulated by microRNAs

MicroRNA(MiRNA)是一类作为基因表达负调控因子的小RNA。人类和小鼠的基因组分别编码超过1400和700个miRNAs。大多数细胞通路被认为是由miRNAs调控的，一些miRNAs被认为参与了化学物质毒性效应的发生。在本研究中，我们开发了一种增加靶向miRNAs功能的方法；给予miRNAs可以增加其功能形式的丰度。

MicroRNA(miRNA) is a class of small RNA that functions as a negative regulator of gene expression. Human and mouse genomes encode over 1, 400 and 700 miRNAs, respectively. Most of the cellular pathways are considered to be modulated by miRNAs, and some miRNAs have been implicated to be involved in the onset of toxic effects of chemicals. In the present study, we developed a method to increase the function of targeted miRNAs ; administration of miRNAs could increase the abundance of their functional form.