Creation of the biological flocculant and inorganic capture protein for environmental purification

用于环境净化的生物絮凝剂和无机捕获蛋白的创建

• 批准号: 23651078
• 负责人: HIROSHI Oyama
• 金额: $ 1.83万
• 依托单位: Setsunan University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23651078/
• 关键词: 濁水浄化; タンパク質; モリンガ; 凝集; 組換え; 種子; 金属

We determined the nucleotide sequence of the structural gene of the protein having an excellent flocculation capacity contained in the seed of a tropical tree, the Moringa oleifera. The protein coagulates soil particle and purifies turbid water. Compared with its deduced amino acid sequence and protein sequences of S and L subunits, we predicted that the Moringa flocculation protein is composed of signal peptide, propeptide, S subunit and linker peptide. We succeeded in the gene expression of S subunit and of the S and L subunits including linker peptide. Based on this result, the construction of the purification system of turbid water and the development of metal capture protein may be enabled.

我们确定了一种热带树木辣木种子中含有的具有良好絮凝能力的蛋白质结构基因的核苷酸序列。蛋白质使土壤颗粒凝固，净化浑浊水。通过对辣木絮凝蛋白的氨基酸序列和S、L亚基的蛋白质序列进行比较，预测其由信号肽、前肽、S亚基和连接肽组成。我们成功地表达了S亚基以及S和L亚基包括连接肽的基因。在此基础上，为浑浊水净化系统的构建和金属捕获蛋白的开发奠定了基础。

项目成果

Altered gene expression profiles associated with enhanced skin inflammation induced by 12-O-tetradecanoylphorbol-13-acetate in streptozotocin-diabetic mice

链脲佐菌素糖尿病小鼠中与 12-O-十四烷酰佛波醇-13-乙酸酯诱导的皮肤炎症增强相关的基因表达谱改变

• 发表时间: 2013
• 期刊: Int Immunopharmacol
• 影响因子: 5.6
• 作者: Iba Y;Watanabe K;Ozaki K;Aozasa O;Ishizawa K;Matsuura T;Oyama H;Masukawa T.
• 通讯作者: Masukawa T.

Visualization of mitochondria in living cells with a genetically encoded yellow fluorescent protein originating from a yellow-emitting luminous bacterium.

使用源自黄色发光细菌的基因编码黄色荧光蛋白对活细胞中的线粒体进行可视化。

• DOI: 10.1039/c3pp25360k
• 发表时间: 2013
• 期刊: Photochem Photobiol Sci.
• 作者: Karatani H;(他8 名、8 番目)
• 通讯作者: (他8 名、8 番目)

Subcellular compartmentation of strontium and zinc in mulberry idioblasts in relation to phytoremediation potential

• DOI: 10.1016/j.envexpbot.2012.06.001
• 发表时间: 2013
• 期刊: Environmental and Experimental Botany
• 影响因子: 5.7
• 作者: H. Katayama;N. Banba;Y. Sugimura;M. Tatsumi;S. Kusakari;H. Oyama;A. Nakahira

細菌由来のオリゴペプチダーゼＢの基質阻害

细菌寡肽酶 B 的底物抑制

• 作者: Malik Suliman Mohamed Mustafa;中嶋義隆;尾山廣;芳本忠;小山裕也;大河原晋;岩田修永;伊藤潔
• 通讯作者: 伊藤潔

Covalent Chromatography for Chymotrypsin-like Proteases Using a Diphenyl 1-Amino-2-phenylethylphosphonate Derivative

使用 1-氨基-2-苯乙基膦酸二苯酯衍生物对胰凝乳蛋白酶样蛋白酶进行共价色谱分析

• 发表时间: 2013
• 期刊: Journal of Biological Macromolecules
• 作者: Shin Ono;Junya Murai;Seigo Furuta;Kazuya Doike;Fumie Manzaki;Toshiaki Yoshimura;Hirofumi Kuroda;Masahito Umezaki;Hiroshi Oyama
• 通讯作者: Hiroshi Oyama