Elucidation of signaling pathway with visualization of redox stateduring nitrogen starvation and diseases accompanying intra-cellular reduction

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In this project we aimed to elucidate the mechanism of intra-cellular reduction in model cell lines of a hereditary disease or in yeast cells exposed to nutrientstarvation (including nitrogen starvation), by utilizing a sensor protein of intra-cellular redox state termed Redoxfluor which we had developed. As a result we found that intra-cellular reduction observed in CHO cell lines of peroxisomal disease had a correlation with the level of very-long chain fatty acid. Furthermore, we discovered that thioredoxin proteins are important for the maintenance of cellular redox in yeast cells exposed to both heat stress and nutrient-deprivation.