Inhibition of biofilm formation focused on de novo biosynthesis of purine nucleotides

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Role of de novo biosynthesis of purine nucleotides on biofilm formation by Pseudomonas fluorescens Pf0-1 was investigated. Analysis of knockout strains revealed that disruption of eight genes in the pathway from phosphoribosyl-1-pyrophosphate to inosine 5’-monophosphate resulted in significant decrease in biofilm formation. Determination of numbers of colony forming units for the mutant strains indicated that the majority of viable cells were found in biofilm. Inhibition of this biosynthetic pathway was detrimental to bacterial growth in the medium, but the mutant strains were still able to form biofilm.