Nanoscale imaging of biocatalytic activity with redox-responsive fluorescent probes

酶和无机纳米颗粒等纳米催化剂在（生物）化学合成和能源生产中具有潜在的和已被证实的应用。在这项研究中，我们开发了新的荧光探针，用于单个纳米催化剂上的分子吸附和界面电子转移反应。合成的基于 BODIPY 的荧光探针具有一个或两个磺酸基和一个二硝基苯基作为氧化还原反应位点，用于在单颗粒、单分子水平上研究催化剂上的光诱导电子转移反应。我们还应用基于单分子的超分辨率荧光显微镜和儿茶酚修饰的 BODIPY 来识别金属氧化物表面上有效吸附位点的位置。

Nano-sized catalysts such as enzymes and inorganic nanoparticles have both potential and demonstrated applications for use in (bio)chemical synthesis and energy production. In this study, we developed new fluorescence probes for molecular adsorption and interfacial electron transfer reactions on individual nanocatalysts. The synthesized BODIPY-based fluorescence probes, which have one or two sulfonate groups and a dinitrophenyl group as a redox reaction site, were used to investigate the photoinduced electron transfer reactions over catalysts at the single-particle, single-molecule levels. We also applied single-molecule-based super-resolution fluorescence microscopy with catechol-modified BODIPY to identify the locations of effective adsorption sites on metal oxide surfaces.