Technical development of selective observation of proteins inliving cells by using ultraviolet resonance Raman spectroscopy

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In order to elucidate functional mechanism based on molecular structure of proteins, it is important to study not only purified proteins but also proteins in living cells. In this project, we developed measurement techniques to selectively observe Raman spectra of proteins in living cells with high sensitivity. We investigated dependences of overexpressed proteins and extent of overexpression on ultraviolet resonance Raman spectra of living cells.