Construction of Artificial Biocatalysts containing Non-natural Dimetallic Active Site

金属酶在温和的条件下催化各种反应，包括甲烷的羟基化和固氮，这些都是合成催化剂难以实现的。因此，这些金属酶的活性部位受到了化学家的关注。在本项目中，构建了含有非天然双金属活性中心的人工生物催化剂，对活性中心的第一配位壳进行了重新设计，并对第二配位壳进行了重新设计。对合成的人工生物催化剂的结构和功能进行了研究。

Metalloenzymes catalyze variety of reactions including hydroxylation of methane and nitrogen fixation, which are difficult for synthetic catalysts, in mild conditions. Thus, the active site of these metalloenzyme has been attracted the chemists. In this project, the artificial biocatalysts containing non-natural dimetallic active sites were constructed.The first coordination shell of the active site was reengineered and the second coordination shell was redesigned. The structure and function of the resultant artificial biocatalysts were studied.