Development of activation technology for cell-free system under microwave irradiation

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New technology of protein expression is necessary for protein crystal structure, biomaterial and medical science. The cell free protein expression system is expected to lead the future bioengineering. To achieve acceleration of activity of enzymatic reactions, we prepared an original microwave applicator for microliter reaction scale. In this study, expression of GFP protein and DNA polymerization were demonstrated by using resonant cavity type of microwave applicator. As a result, we confirmed the microwave effectto enzymatic reactions.