Characterization of USP54 and its splicing variant in glioblastoma cells

胶质母细胞瘤细胞中 USP54 及其剪接变体的表征

• 批准号: 23791591
• 负责人: HASEGAWA Hitoki
• 金额: $ 2.75万
• 依托单位: Nagoya University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23791591/
• 关键词: グリオーマ; 細胞分裂; 細胞周期; 脱ユビキチン化酵素; USP54; 細胞質分裂

We found that USP54 was phosphorylated during mitotic phase. USP54 is localized at cell-cell junction, which is dependent on an association with YWHAE protein. USP54 stabilize ZO-1 protein expression and has important role in cell-cell junction. We identified splicing variant of USP54, whose expression induced aberrant cell morphology and mitosis in glioblastoma cells.

我们发现USP54在有丝分裂期被磷酸化。USP54定位于细胞-细胞交界处，依赖于与YWHAE蛋白的结合。USP54稳定ZO-1蛋白表达，在细胞间连接中发挥重要作用。我们鉴定了USP54的剪接变异体，它的表达诱导了胶质母细胞瘤细胞的形态异常和有丝分裂。

项目成果

PLK1 controls cytokinetic furrowing through phosphorylation of an actin/myosin binding protein, supervillin

PLK1 通过肌动蛋白/肌球蛋白结合蛋白 supervillin 的磷酸化来控制细胞因子皱褶

• 发表时间: 2012
• 作者: 長谷川仁紀;千賀威
• 通讯作者: 千賀威

Forced expression of miR-143 represses ERK5/c-Myc and p68/p72 signaling in concert with miR-145 in gut tumors of Apc(Min) mice.

• DOI: 10.1371/journal.pone.0042137
• 发表时间: 2012
• 期刊: PloS one
• 影响因子: 3.7
• 作者: Takaoka Y;Shimizu Y;Hasegawa H;Ouchi Y;Qiao S;Nagahara M;Ichihara M;Lee JD;Adachi K;Hamaguchi M;Iwamoto T
• 通讯作者: Iwamoto T

Misshapen-like kinase 1 (MINK1) Is a Novel Component of Striatin-interacting Phosphatase and Kinase (STRIPAK) and Is Required for the Completion of Cytokinesis

• DOI: 10.1074/jbc.m112.372342
• 发表时间: 2012-07-20
• 期刊: JOURNAL OF BIOLOGICAL CHEMISTRY
• 影响因子: 4.8
• 作者: Hyodo, Toshinori;Ito, Satoko;Senga, Takeshi
• 通讯作者: Senga, Takeshi

ALX1 Induces Snail Expression to Promote Epithelial-to-Mesenchymal Transition and Invasion of Ovarian Cancer Cells

• DOI: 10.1158/0008-5472.can-12-2377
• 发表时间: 2013-03-01
• 期刊: CANCER RESEARCH
• 影响因子: 11.2
• 作者: Yuan, Hong;Kajiyama, Hiroaki;Senga, Takeshi
• 通讯作者: Senga, Takeshi