Comprehensive analysis of glioma LOH regions using next-generation sequencing

使用新一代测序对神经胶质瘤 LOH 区域进行综合分析

• 批准号: 23791608
• 负责人: HATA Nobuhiro
• 金额: $ 2.83万
• 依托单位: Kyushu University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23791608/
• 关键词: 脳腫瘍; 神経膠腫; next-generation sequencing; LOH; IDH1; IDH2; glioma; glioblastoma; cancer stem cell; micro RNA; genome sequencer; chromosome 10; 1p/19q LOH

Mutation analyses on patient-derived glioma tissues: IDH1/2 mutations and LOH on 1p, 19q, 10, and 17p were analyzed on 162 patient-derived glioma tissues using conventional capillary sequencer. IDH1 mutant primary GBMs showed distinct clinical specificities. By IDH mutation and 1p/19q LOH analyses, grade III oligodendroglial tumors were divided 3 subtypes showing different clinical outcomes. Comprehensive mutation analysis on glioma cancer stem cells using next-generation sequencer: Four patient-derived GBM sphere cultures were analyzed by amplicon PCR using a Truseq Amplicon Cancer Panel, followed by next-generation sequencing using a Miseq instrument (Illumina). Bioinformatics analysis was performed using 'CLC GenomicsWorkbench' software

患者源性胶质瘤组织的突变分析：使用常规毛细管测序仪分析了162例患者源性胶质瘤组织的IDH 1/2突变和1 p、19 q、10和17 p上的洛杂合性缺失。IDH 1突变型原发性GBM显示出不同的临床特异性。通过IDH突变和1 p/19 q洛缺失分析，将III级少突胶质细胞瘤分为3个亚型，表现出不同的临床预后。使用下一代测序仪对神经胶质瘤癌症干细胞进行综合突变分析：使用Truseq Amplicon Cancer Panel通过扩增子PCR分析四种患者来源的GBM球体培养物，然后使用Miseq仪器（Illumina）进行下一代测序。使用'CLC GenomicsTools'软件进行生物信息学分析

项目成果

Long-term outcome of patients with oligodendroglial tumors treated up-front

少突胶质细胞瘤患者预先治疗的长期结果

• 发表时间: 2013

Long-term outcome of patients with oligodendroglial tumors treated up-front with PAV therapy

预先接受 PAV 治疗的少突胶质细胞瘤患者的长期结果

• 发表时间: 2013
• 作者: 秦 暢宏

ダイレクトシークエンス法によるIDH1 mutation解析：グリオーマ126例での検討

直接测序法IDH1突变分析：126例胶质瘤病例研究

• 发表时间: 2011
• 作者: 秦 暢宏

Molecular biomarkers of glioblastoma: current targets and clinical implications

胶质母细胞瘤的分子生物标志物：当前目标和临床意义

• 发表时间: 2012
• 期刊: Current Biomarker Findings
• 作者: Yoshimoto K;Mizoguchi M;Hata N;et. al.
• 通讯作者: et. al.

IDH1 /2変異解析簡便化の試み

尝试简化 IDH1/2 突变分析

• 发表时间: 2012