Involvement of calcitonin gene-related peptide familyand TRPV1 in various pain states

降钙素基因相关肽家族及TRPV1参与多种疼痛状态

• 批准号: 23791698
• 负责人: ISHIDA Kumiko
• 金额: $ 2.66万
• 依托单位: Shinshu University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23791698/
• 关键词: CGRPファミリー; TRPV1; CGRP; アドレノメデュリン; 血管新生

We studied the involvement of TRPV1 and αCGRP in incisional pain model and inflammatory pain model by using αCGRP knockout mice and CGRP receptor antagonist. Our study suggested that1) αCGRP was involved in inflammatory pain but not in incisional pain model2) αCGRP was involved in spontaneous pain and thermal hyperalgesia in inflammatory pain model in the dorsal horn spinal cord, and3) αCGRP was involved in some strong and long lasting pain. Thus, antagonists of CGRP receptors may be useful for some kinds of pain such as inflammatory pain.CGRP was mainly expressed in TRPV1-positive afferents, and spinal release of CGRP following activation of TRPV1-positive afferents would be one of mechanism underlying such inflammatory induced spontaneous pain and thermal hyperalgesia.

本实验利用α基因敲除小鼠和α受体拮抗剂，研究了TRPV1和CGRP在切口痛模型和炎症性疼痛模型中的作用。结果提示：1)α降钙素基因相关肽参与炎性痛模型，而不参与切口性疼痛模型；2)α降钙素基因相关肽参与炎性痛模型的自发痛和热痛敏反应；3)α降钙素基因相关肽参与一些强而持久的疼痛。因此，CGRP受体拮抗剂可能对炎症性疼痛等疼痛有一定的治疗作用。CGRP主要在TRPV1阳性传入神经元中表达，激活TRPV1阳性传入神经元后脊髓释放CGRP可能是炎症性自发性疼痛和热痛敏的机制之一。

项目成果

Long-term but not short-term spontaneous pain may trigger release of αCGRP within the spinal cord in an inflammatory pain model.

在炎性疼痛模型中，长期而不是短期的自发性疼痛可能会触发脊髓内 αCGRP 的释放。

• 发表时间: 2012
• 作者: Ishida K;Kawamata T;Shindo T;Kawamata M
• 通讯作者: Kawamata M

αCGRP is involved in inflammation- but not incision induced thermal hyperalgesia

αCGRP 参与炎症，但不参与切口诱导的热痛觉过敏

• 发表时间: 2011
• 作者: Ishida K;Kawamata T;Shindo T;Ishida T;Kawamata M
• 通讯作者: Kawamata M

各種疼痛モデルにおけるαCGRPの関与

αCGRP 参与各种疼痛模型

• 发表时间: 2011
• 作者: 石田公美子;川股知之;田中聡;川真田樹人
• 通讯作者: 川真田樹人

αCGRP is involved in inflammation- but not incision- induced thermal hyperalgesia

αCGRP 参与炎症而非切口诱导的热痛觉过敏

• 发表时间: 2011
• 作者: 石田公美子;川股知之;田中聡;川真田樹人;石田公美子;石田公美子
• 通讯作者: 石田公美子

TRPV1-positive afferents and IB4-binding afferents sense different modalities of hyperalgesia in an incisional pain model

TRPV1 阳性传入神经和 IB4 结合传入神经在切口痛模型中感知不同的痛觉过敏模式

• 发表时间: 2012
• 作者: Kawamata T;Ishida KM;Tanaka H;Kawamata Y;Kawamata M
• 通讯作者: Kawamata M