Analysis of effects of anesthetic agent on rat somatosensory cortexusing in vivo patch clamp recording

本研究采用在体全细胞膜片钳技术，记录大鼠后爪感受野对机械刺激的反应，记录开始后静脉注射异丙酚、戊巴比妥、瑞芬太尼或氯胺酮。我们通过分析捏刺激引起的反应来评价这些麻醉剂的效果

In this project, in vivo whole-cell patch-clamp recordings were performed from primary somatosensoryneurons which responded to the mechanical stimuli to the receptive field of the hind paw area.Propofol, pentobarbital, remifentanil or ketaminewas administered intravenously to the rat after starting recordings. We evaluated the effect of these anesthetic agents by analyzing the responses evoked by pinch stimuli