Mechanisms of high-fat induced prostate cancer progression through Monocyte Chemotactic Protein-1 secreted by adipose tissue

高脂通过脂肪组织分泌的单核细胞趋化蛋白-1诱导前列腺癌进展的机制

• 批准号: 23791739
• 负责人: SHINTARO Narita
• 金额: $ 2.33万
• 依托单位: Akita University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23791739/
• 关键词: 前立腺癌; 肥満; 高脂肪; Monocyte Chemotactic Protein-1; MCP-1; 高脂肪食

We investigated the effects of different diets on prostate cancer cell growth and the relevant circulating factors including serum insulin, growth factors, and inflammatory cytokines using the in vivo and ex vivo model. The tumor growth of prostate cancer LNCaP xenograft was significantly higher in the HFD group than in the HCD and control diet (CD) groups. The mean level of the serum monocyte chemoattractant protein-1 (MCP-1) in the HFD group was significantly higher than that in the HCD and CD groups. The mRNA levels of CC chemokine receptor 2 (CCR2), which is an MCP-1 receptor, and the expression of activated Akt were the highest in the HFD group. Furthermore, serum from HFD-fed mice enhanced the proliferation of two PCa cells and CCR2 knockdown inhibited HFD-induced proliferation of LNCaP cells. In conclusion, an HFD enhanced prostate cancer cell growth more strongly than an HCD or CD. MCP-1/CCR2 signaling may be involved in an HFD-induced prostate cancer progression.

我们采用体内和离体模型研究了不同饮食对前列腺癌细胞生长和相关循环因子（包括血清胰岛素、生长因子和炎症因子）的影响。HFD组前列腺癌LNCaP异种移植瘤的肿瘤生长明显高于HCD和对照饮食（CD）组。HFD组大鼠血清单核细胞趋化蛋白-1 （MCP-1）平均水平显著高于HCD和CD组。MCP-1受体CC趋化因子受体2 （CCR2）的mRNA水平和活化Akt的表达在HFD组中最高。此外，hfd喂养小鼠的血清增强了两种PCa细胞的增殖，CCR2敲低抑制了hfd诱导的LNCaP细胞的增殖。总之，HFD比HCD或CD更能促进前列腺癌细胞的生长。MCP-1/CCR2信号通路可能参与了HFD诱导的前列腺癌进展。

项目成果

前立腺癌進展における長鎖脂肪酸パルミチンの役割

长链脂肪酸棕榈汀在前列腺癌进展中的作用

• 发表时间: 2012
• 作者: 黄明国;成田伸太郎,井上高光,土谷順彦,佐藤滋,羽渕友則
• 通讯作者: 成田伸太郎,井上高光,土谷順彦,佐藤滋,羽渕友則

Prostate cancer progression under high fat diet is enhanced by the MCP-1/CCR2 signaling

MCP-1/CCR2 信号传导可促进高脂肪饮食下前列腺癌的进展

• 发表时间: 2011
• 作者: Huang;M.;Narita;S.;Numakura;K.;Tsuruta;H.;Saito;M.;Inoue;T.;Horikawa;Y.;Tsuchiya;N.;Habuchi;T.
• 通讯作者: T.

Overexpression of Fn14 promotes androgen-independent prostate cancer progression through MMP-9 and correlates with poor treatment outcome

• DOI: 10.1093/carcin/bgr182
• 发表时间: 2011-11-01
• 期刊: CARCINOGENESIS
• 影响因子: 4.7
• 作者: Huang, Mingguo;Narita, Shintaro;Habuchi, Tomonori
• 通讯作者: Habuchi, Tomonori

サイトカインシグナルを介した高脂肪食摂取下の前立腺癌増殖機序の解明(ヤングリサーチグラント受賞者記念講演)

阐明细胞因子信号介导的高脂肪饮食摄入下前列腺癌生长的机制（青年研究基金获奖者纪念讲座）

• 发表时间: 2011
• 作者: Huang;M.;Narita;S.;Numakura;K.;Tsuruta;H.;Saito;M.;Inoue;T.;Horikawa;Y.;Tsuchiya;N.;Habuchi;T.;成田伸太郎
• 通讯作者: 成田伸太郎

A High-Fat Diet Enhances Proliferation of Prostate Cancer Cells and Activates MCP-1/CCR2 Signaling

• DOI: 10.1002/pros.22531
• 发表时间: 2012-12-01
• 期刊: PROSTATE
• 影响因子: 2.8
• 作者: Huang, Mingguo;Narita, Shintaro;Habuchi, Tomonori
• 通讯作者: Habuchi, Tomonori