Amelioration of Acute Tubular Necrosis in Ischemic Acute Renal Failure was Impaired in Mice Lucking Hypoxia Inducible Factor-1α Gene

利用HIF-1αkd小鼠和WILD型小鼠的IRI模型肾脏样本进行了DNA微阵列分析，并进行了彻底的基因表达分析，发现存在一些被认为与应激反应和免疫反应相关的基因，细胞组织修复。我们对其中的“CHAC1”基因进行了关注和分析。通过HK-2细胞体外证实实验，实时PCR证实CHAC1基因表达明显升高，Western blot证实CHAC1基因在蛋白水平上表达相似。基于这些结果，CHAC1对HIF-1α的诱导作用在CHIP实验中得到了证实，但目前还在进一步研究中。

As a result of DNA microarrays were performed using kidney sample of the IRI model of HIF-1αkd mice and WILD type mice, and having analyzed exhaustive gene expression analysis, some genes which were thought to be associated with a stress reply and an immune response, cellular tissue repair were found to be present. We paid its attention to "CHAC1" gene of those and pushed forward an analysis. By the in vitro confirmation experiment using HK-2 cells, it could be confirmed that the expression of CHAC1 gene rose in real time PCR conspicuously and was able to confirm similar expression ata protein level in Western blot. Based on these results, the inductivity by HIF-1α of CHAC1 is confirmed using CHIP assay, but is progressing now.