Identification of novel urine biomarkers using molecules which was Specifically expressed in bladder cancer

作为膀胱癌新的诊断手段，我们尝试建立新的膀胱癌尿液生物标志物。尽管我们分析了MPHOSPH1和DEPDC1的基因表达水平，但白细胞的污染影响了结果。因此，我们利用CD13和CD31的表达水平对污染水平进行了校正。结果表明，以CD31表达为校正因子时，膀胱癌患者MPHOSPH1和DEPDC1的表达水平均高于正常对照组。蛋白质表达水平也显示出类似的结果。

As new diagnostic tools of bladder cancer, we attempted to establish novel urine biomarker for the bladder cancer. Although we analyzed gene expression levels of MPHOSPH1 and DEPDC1, contamination of white blood cells affected the results. Therefore we corrected contamination level using expression levels of CD13 and CD31. As a results, when we use CD31 expression as correction factor, Expression levels of MPHOSPH1 and DEPDC1 of bladder cancer patients were higher than that of normal controls. Protein expression level showed similar result.