Development of a Hybrid Three-Dimensional Culture Method in Regenerative Medicine

脂肪源性干细胞（ASC）和成纤维细胞各自促进气管上皮层的再生。在这项研究中，我们创建了细胞分层排列的3D架构（3D-A），以便同时利用ASC和成纤维细胞的优点。在将3D-A移植到大鼠的气管损伤模型中后，我们能够证实功能性气管上皮层的再生在比单独移植ASC或成纤维细胞更短的时间内接近其正常形态。这种混合3D培养方法的发展似乎能够为气管再生医学做出巨大贡献。

Adipose Derived Stem Cells (ASC) and fibroblasts each promote the regeneration of the tracheal epithelial layer. In this study, we created a 3D Architecture of cell layered arrangement (3D-A) in order to simultaneously make use of the strong points of ASC and fibroblasts. Upon grafting of 3D-A into the tracheal injury model of a rat, we were able to confirm the regeneration of a functional tracheal epithelial layer to near its normal morphology in a shorter time than with grafting of ASC or fibroblasts alone. The development of this hybrid 3D culture method seems to be capable of contributing greatly to trachea regenerative medicine.