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Immunological responses against human papilloma virus and human papilloma virus induced laryngeal cancer

针对人乳头瘤病毒和人乳头瘤病毒诱发的喉癌的免疫反应

基本信息

批准号：
23791949
负责人：
CHITOSE Syunichi
金额：
$ 1.58万
依托单位：
Kurume University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Young Scientists (B)
财政年份：
2011
资助国家：
日本
起止时间：
2011 至 2012
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23791949/
关键词：
乳頭腫喉頭癌化防御反射感染防御 HPV陽性喉頭癌

项目摘要

Objective: This study aimed to clarify the local immune status in the larynx in the presence of infection or carcinogenesis associated with human papilloma virus. Methods: Cytological samples (for human papilloma virus detection) and laryngeal secretions (for immunoglobulin assessment) were obtained from 31 patients with laryngeal disease, during microscopic laryngeal surgery. On histological examination, 12 patients had squamous cell carcinoma, four had laryngeal papilloma and 15 had other benign laryngeal disease. Cytological samples were tested for human papilloma virus DNA using the Hybrid Capture 2 assay. Results: High risk human papilloma virus DNA was detected in 25 per cent of patients (three of 12) with laryngeal cancer. Low risk human papilloma virus DNA was detected only in three laryngeal papilloma patients. The mean laryngeal secretion concentrations of immunoglobulins M, G and A and secretory immunoglobulin A in human papilloma virus DNA positive patients were more than t … More wice those in human papilloma virus DNAnegative patients. A statistically significant difference was observed between the secretory immunoglobulin A concentrations in the two groups. Patients with laryngeal cancer had higher laryngeal secretion concentrations of each immunoglobulin type, compared with patients with benign laryngeal disease. The study assessed the mean laryngeal secretion concentrations of each immunoglobulin type in the 12 laryngeal cancer patients, comparing human papilloma virus DNA positive patients (n = 3) and human papilloma virus DNA negative patients (n = 9); the mean concentrations of immunoglobulins M, G and A and secretory immunoglobulin A tended to be greater in human papilloma virus DNA positive cancer patients, compared with human papilloma virus DNA negative cancer patients. Conclusion: These results suggest that the local laryngeal immune response is activated by infection or carcinogenesis due to human papilloma virus. The findings strongly suggest that secretory IgA has inhibitory activity against infection or carcinogenesis associated with human papilloma virus in thelarynx. Less

目的：本研究的目的是阐明局部免疫状态在喉的存在感染或癌变相关的人乳头瘤病毒。研究方法：细胞学样本（用于人乳头状瘤病毒检测）和喉分泌物（用于免疫球蛋白评估），从31例喉部疾病，在显微镜喉手术。组织学检查，12例为鳞状细胞癌，4例为喉乳头状瘤，15例为其他喉良性疾病。使用Hybrid Capture 2检测试剂盒检测细胞学样本中的人乳头瘤病毒DNA。结果：25%的喉癌患者（12例中有3例）检测到高危型人乳头状瘤病毒DNA。仅在3例喉乳头状瘤患者中检测到低危型人乳头状瘤病毒DNA。人乳头瘤病毒DNA阳性患者喉分泌物中免疫球蛋白M、G、A及分泌型免疫球蛋白A的平均浓度均高于正常对照组。 ...更多信息而人乳头瘤病毒DNA阴性者则明显高于阴性者。在两组中的分泌型免疫球蛋白A浓度之间观察到统计学显著差异。喉癌患者喉分泌物中每种免疫球蛋白的浓度均高于喉良性疾病患者。该研究评估了12例喉癌患者中每种免疫球蛋白类型的平均喉分泌物浓度，比较了人乳头瘤病毒DNA阳性患者（n = 3）和人乳头瘤病毒DNA阴性患者（n = 9）;免疫球蛋白M、G和A以及分泌型免疫球蛋白A的平均浓度在人乳头瘤病毒DNA阳性癌症患者中倾向于更高，与人乳头瘤病毒DNA阴性的癌症患者相比。结论：人乳头瘤病毒感染或癌变可激活喉局部免疫反应。这些发现强烈提示分泌型伊加对喉中与人乳头状瘤病毒相关的感染或癌变具有抑制活性。少