Validity of blood tests and use of genetic profiles for detection of unknown diagnostic items

血液检测的有效性以及使用基因图谱检测未知诊断项目的有效性

• 批准号: 23792083
• 负责人: AKITOMI Shinji
• 金额: $ 2.58万
• 依托单位: Iwate Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23792083/
• 关键词: sepsis; mRNA; miRNA; microarray; 敗血症; m-RNA; mi-RNA; 治療マーカー; 敗血症性ショック; 遺伝子プロファイル; PMX

We carried out gene profiling before and after treatment in patients with severe sepsis. The results obtained were evaluated and compared between those who recovered from septic shock with treatments for severe sepsis, such as endotoxin adsorption therapy (polymyxin-B direct hemoperfusion) and those who were in prolonged shock without responding to treatment. Regardless of shock state improvement, 10 types of miRNA increased, and 8 types decreased, with intensive care. Among them, there were 7 types of miRNA in cases with blood pressure elevation in response to treatment. Regarding these 7 types, we analyzed the target genes of miRNA that was changed in patients with definitely elevated blood pressure.We confirmed 94 types of blood-related genes contributing to blood pressure elevation. At present, we are analyzing the pathways of mRNA and miRNA.

我们对严重脓毒症患者治疗前后进行了基因谱分析。对接受内毒素吸附治疗（多粘菌素- b直接血液灌流）等严重脓毒症治疗后脓毒性休克恢复的患者和对治疗无反应的长期休克患者进行评价和比较。无论休克状态是否改善，随着重症监护，10种miRNA增加，8种miRNA减少。其中，治疗后血压升高的病例有7种miRNA。针对这7种类型，我们分析了在明确血压升高的患者中发生改变的miRNA靶基因。我们确认了94种与血压升高有关的血液相关基因。目前，我们正在分析mRNA和miRNA的通路。