The effect of glycosyltransferase GnT-V in the development and progression of oral cancer and evaluation by PET.

糖基转移酶GnT-V在口腔癌发生、进展中的作用及PET评估。

• 批准号: 23792307
• 负责人: ONODERA Makiko
• 金额: $ 2.66万
• 依托单位: Hokkaido University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23792307/
• 关键词: GnT-V; PET; HIF-1; 口腔がん; 糖鎖; 低酸素; HIF-1α

The pattern and expression of cell surface sugar chains greatly alter during the carcinogenesis and play important role in tumor progression. To clarify the role of glycosyltrasferase GnT-V in cancer development we examined the expression of GnT-V in the oral cancer association with the PET scan. Both GnT-V mRNA and protein are strongly enhanced in oral cancer compared with normal oral mucosa tissue. In the group with high SUV on FDG-PET and FMISO-PET hypoxia imaging, GnT-V and HIF-1α had tendency to express higher. The expression and function of GnT-V may increase in hypoxia condition. By treatment the human oral squamous cell carcinoma cell line SAS with swainsonine, an inhibitor of N-glycan processing, cell motility was reduced significantly. These results showed that the glycosylation by GnT-V is associate with cell motility and invasion enhancement in oral cancer and related in oral cancer progression.

细胞表面糖链的模式和表达在肿瘤发生过程中发生了很大的变化，并在肿瘤的进展中起着重要的作用。为了阐明糖基转移酶GnT-V在癌症发展中的作用，我们用PET扫描检测了GnT-V在口腔癌中的表达。与正常口腔粘膜组织相比，GnT-V mRNA和蛋白在口腔癌中均强烈增强。在FDG-PET和FMISO-PET乏氧显像SUV值高的组中，GnT-V和HIF-1α有表达增高的趋势。GnT-V在缺氧条件下表达增强，功能增强。N-聚糖加工抑制剂苦马豆素处理人口腔鳞状细胞癌细胞系SAS，细胞运动能力明显降低。这些结果表明，GnT-V的糖基化与口腔癌细胞的运动和侵袭增强有关，并与口腔癌的进展有关。

项目成果

FDG-PET in combination with serum- soluble interleukin-2 receptor to predict prognosis in patients with head and neck malignant lymphoma

FDG-PET联合血清可溶性白细胞介素2受体预测头颈恶性淋巴瘤患者预后

• 发表时间: 2011
• 作者: Y. Kitagawa;Makiko Kyan-Onodera
• 通讯作者: Makiko Kyan-Onodera

FDG-PET in combination with serum-soluble interleukin-2 receptor (sIL-2R) to predict prognosis in patients with head and neck malignant lymphoma

FDG-PET联合血清可溶性白细胞介素2受体(sIL-2R)预测头颈部恶性淋巴瘤患者的预后

• 发表时间: 2011
• 作者: Yoshimasa Kitagawa;Makiko Kyan-Onodera
• 通讯作者: Makiko Kyan-Onodera

Role of FDG-PET in evaluating different 4 types of chronic osteomyelitis of jaws in combination with 3-phase bone scintigraphy

FDG-PET联合三相骨显像对4种不同类型颌骨慢性骨髓炎的评估作用

• 发表时间: 2012
• 作者: Y. Kitagawa;Makiko Kyan-Onodera
• 通讯作者: Makiko Kyan-Onodera

Role of FDG-PET in evaluating different 4 types of chronic osteomyelitis of jaws in combination with 3-phase bone scintigraphy.

FDG-PET联合三相骨显像在评估不同4种颌骨慢性骨髓炎中的作用

• 发表时间: 2012
• 作者: Yoshimasa Kitagawa;Makiko Kyan-Onodera
• 通讯作者: Makiko Kyan-Onodera