Bone regeneration by using mononuclear cells derived from peripheral blood with homing factor

利用具有归巢因子的外周血单核细胞进行骨再生

• 批准号: 23792361
• 负责人: OHBA Seigo
• 金额: $ 2.75万
• 依托单位: University of Fukui (2012)Nagasaki University (2011)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23792361/
• 关键词: PRP; 再生; MNC; レプチン; 単核細胞; 骨再生; ホーミング

There are certain cells which have multilineage potentials in the mononuclear cells of peripheral blood. These cells with the scaffold(B-TCP) were transplanted on the calvalia of mice as an onlay, resulting in synthesizing new bone formation. The amount of new bone was more than the scaffold without cells. Moreover, the bone induction potency was comparable as the bone marrow-derived mononuclear cells.The mesenchymal stem cells extracted from the marrow of the femur of mice were cultured. These cells were exposed by missed of leptin and BMP-2, which dose was too low to induce more osteoblastogenesis by solely, and the alkaline phosphatase activity was calculated. Moreover, when the gelatin containing leptin and scaffold (B-TCP) was transplanted to the defect region with a clinical size, created to the calvarial bone of a mouse, induced bone regeneration.

外周血单核细胞中有一些细胞具有多系电位。将这些细胞与支架（B-TCP）一起移植到小鼠足骨上，形成新骨。新骨的数量比没有细胞的支架要多。此外，其骨诱导能力与骨髓来源的单个核细胞相当。从小鼠股骨骨髓中提取间充质干细胞进行培养。分别用过低剂量的瘦素和BMP-2单独暴露于成骨细胞后，计算碱性磷酸酶活性。此外，当将含有瘦素和支架（B-TCP）的明胶移植到具有临床尺寸的小鼠颅骨缺损区域时，可诱导骨再生。

项目成果

In vivo comparison of the bone regeneration capacity of human bone marrow concentrates vc

人骨髓浓缩物vc骨再生能力的体内比较

• 发表时间: 2012
• 期刊: Platelet-rich plasma.
• 作者: Zhong;W;Sumita Y;Ohba S;Kawasaki T;Nagai K;Ma G;Asahina I
• 通讯作者: Asahina I

骨形成におけるレプチンとBMP-2の相乗効果の検討

检查瘦素和 BMP-2 对骨形成的协同作用

• 发表时间: 2012
• 作者: 本岡菜穂美;大場誠悟;朝比奈泉
• 通讯作者: 朝比奈泉

In vivo comparison of the bone regeneration capability of human bone marrow concentrates vs. platelet-rich plasma.

• DOI: 10.1371/journal.pone.0040833
• 发表时间: 2012
• 期刊: PloS one
• 影响因子: 3.7
• 作者: Zhong W;Sumita Y;Ohba S;Kawasaki T;Nagai K;Ma G;Asahina I
• 通讯作者: Asahina I

Bone regeneration capability of human bone marrow concentrates versus platelet rich plasma

人骨髓浓缩物与富含血小板血浆的骨再生能力

• 发表时间: 2012
• 作者: Mayumi Umebayashi;Weijian Zhong;Yoshinori Sumita;Seigo Ohba;Kazuhiro Nagai;Izumi Asahina
• 通讯作者: Izumi Asahina

Bone Regeneration Capability of Human Bone-Marrow Concentrates vs. Platelet-Rich Plasma.

人骨髓浓缩物与富含血小板血浆的骨再生能力。

• 发表时间: 2012
• 作者: Umebayashi M;Zhong W;Sumita Y;Ohba S;Nagai;Asahina I
• 通讯作者: Asahina I