Preparation and Application of polythiophene/protein conjugate

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Preparations of poly (3,4-ethylenedioxythiophene)/protein (PEDOT/protein) nanoparticle, consisting of the oligomeric PEDOTs and natural proteins were achieved by an oxidative polymerization of 3,4-ethylenedioxythiophene (EDOT) in the presence of partially denatured proteins in an aqueous solution containing 3 M Gdm HCl. From the dynamic light scattering (DLS) and the transmission electron microscope (TEM) observations, the hydrodynamic diameters of obtained nanoparticles were homogeneous by ~ 50 nm. We evaluated the redox potentials of PEDOT/protein nanoparticles using the cyclic voltammetric analyses and the observed redox potentials have a dependence on the choice of natural proteins. The current method to prepare the PEDOT/protein nanoparticle should be applicable to construct various kinds of the PEDOT/biomolecule nanoparticles, leading to the construction of redox-active sensor conjugates enable to use in vitro and in vivo.