Finding the coenzyme A binding sites and mechanism of enantioselectivity of firefly luciferase catalyzed thioesterification reaction

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Firefly luciferase, which is very famous enzyme for bioluminescence reaction, can also catalyze the enantioselective thioesterification reaction toward 2-arylpropanoic acid. The detail catalytic mechanisms for enantioselectivity and/or a binding site of coenzyme A, however, were not disclosed. Thus in this project, we tried to find the binding site for coenzyme A and tried to explain the mechanism of enantioselectivity toward 2-arylpropanoic acid. Finally we achieved to find some important residues for identifying of substrate chirality.