Reverse Glycomic Approach for Development of Simple Diagnostic Method of Antitumor-Drug Resistance

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To develop simple diagnostic method of antitumor-drug resistance, we determined the changes in glycosylation that occur on membrane proteins of drug-resistant T-cell acute lymphoblastic leukemia cell line. Drug-resistant cells showed a significant decrease in sialylated glycans. To ascertain if identical alterations occurred on leukemia cell in the blood of leukemia patients, the analytical conditions for LC-ESI MS of glycan structures were investigated. We established the conditions that could analyze their glycan structures using 3 mL of blood. Moreover, we showed that the lectin recognized sialic acids was useful for investigating a simple diagnostic method of drug-resistance acquisition.