Reverse Glycomic Approach for Development of Simple Diagnostic Method of Antitumor-Drug Resistance

逆糖组学方法开发简单的抗肿瘤耐药性诊断方法

• 批准号: 23770120
• 负责人: NAKANO Miyako
• 金额: $ 3万
• 依托单位: Hiroshima University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23770120/
• 关键词: 糖質; 糖鎖; 癌; 構造解析; 薬物耐性; 白血病; 微小管脱重合阻害薬

To develop simple diagnostic method of antitumor-drug resistance, we determined the changes in glycosylation that occur on membrane proteins of drug-resistant T-cell acute lymphoblastic leukemia cell line. Drug-resistant cells showed a significant decrease in sialylated glycans. To ascertain if identical alterations occurred on leukemia cell in the blood of leukemia patients, the analytical conditions for LC-ESI MS of glycan structures were investigated. We established the conditions that could analyze their glycan structures using 3 mL of blood. Moreover, we showed that the lectin recognized sialic acids was useful for investigating a simple diagnostic method of drug-resistance acquisition.

为了建立简便的肿瘤耐药诊断方法，我们测定了耐药T细胞急性淋巴细胞性白血病细胞系细胞膜蛋白糖基化的变化。耐药细胞显示唾液酸聚糖显著减少。为了确定白血病患者血液中的白血病细胞是否发生了相同的变化，对LC-ESIMS分析条件进行了研究。我们建立了用3毫升血液分析它们的糖链结构的条件。此外，我们还表明，凝集素识别唾液酸有助于研究一种简单的耐药获得的诊断方法。

项目成果

Alterations of glycan structures on cell membrane glycoproteins in microtubule-targeting drug-resistant leukemia cells

微管靶向耐药白血病细胞细胞膜糖蛋白聚糖结构的改变

• 发表时间: 2012
• 作者: Nakano M;Shirai R;Ito J;Takahashi S
• 通讯作者: Takahashi S

Site-specific analyses of N-glycans on haptoglobin in sera of patients with various kinds of gastroenterological cancers

各种胃肠道癌症患者血清中触珠蛋白 N-聚糖的位点特异性分析

• 发表时间: 2012
• 作者: Takahashi S;Amimoto T;Kawamoto S;Ono K;Miyoshi E;Nakano M
• 通讯作者: Nakano M

Drug-resistance inhibits expression of alpha2-6 sialylation on cell membrane glycoproteins

耐药性抑制细胞膜糖蛋白上α2-6唾液酸化的表达

• 发表时间: 2011
• 作者: Nakano M;Ota F;Nakajima K;Taniguchi N;Kavallaris M;Packer N
• 通讯作者: Packer N

Identification of alterations in glycan structure on cell membrane proteins in microtubule-targeting drug-resistant leukemia cells

微管靶向耐药白血病细胞细胞膜蛋白聚糖结构变化的鉴定

• 发表时间: 2012
• 作者: NakanoM;TaniguchiN;Ito J;ShiraiR;KavallarisM;Packer
• 通讯作者: Packer

微小管作用抗癌剤の耐性獲得による白血病細胞膜糖タンパク質糖鎖の構造変化

由于对微管作用抗癌药物的获得性耐药性导致白血病细胞膜糖蛋白糖链的结构变化

• 发表时间: 2012
• 作者: 中の三弥子;白井亮平;伊藤潤;高橋志郎
• 通讯作者: 高橋志郎