Structural studies for the refined reaction mechanism of copper amine oxidase.

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Copper amine oxidases (CAOs) catalyse the oxidation of various aliphatic amines to the corresponding aldehydes, ammonia and hydrogen peroxide. I focused on the structural insights into the catalytic mechanism of a CAO from Arthrobacter globiformis (AGAO) using an ultra-high resolution X-ray crystallography and neutron crystallography method. For the ultra-high resolution X-ray crystallography, the crystal structure of AGAO was determined at 1.08 A resolution in the substrate-free form. Based on the structure, the oxygen pathway to the active site was estimated. On the other hand, on the basis of the structure of the AGAO complexed with irreversible inhibitors, structural insights of the substrate specificity were obtained. Furthermore, a large crystal of AGAO for a neutron crystallography was prepared.