Structural studies for the refined reaction mechanism of copper amine oxidase.

铜胺氧化酶精细反应机制的结构研究。

• 批准号: 23770127
• 负责人: TAKESHI Murakawa
• 金额: $ 2.91万
• 依托单位: Osaka Medical College
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23770127/
• 关键词: トパキノン; 高分解能X線結晶解析; 中性子線結晶構造解析; 超高分解能X腺結晶構造解析; TPQ; アミン酸化酵素; 高分解能; 中性子線; X線

Copper amine oxidases (CAOs) catalyse the oxidation of various aliphatic amines to the corresponding aldehydes, ammonia and hydrogen peroxide. I focused on the structural insights into the catalytic mechanism of a CAO from Arthrobacter globiformis (AGAO) using an ultra-high resolution X-ray crystallography and neutron crystallography method. For the ultra-high resolution X-ray crystallography, the crystal structure of AGAO was determined at 1.08 A resolution in the substrate-free form. Based on the structure, the oxygen pathway to the active site was estimated. On the other hand, on the basis of the structure of the AGAO complexed with irreversible inhibitors, structural insights of the substrate specificity were obtained. Furthermore, a large crystal of AGAO for a neutron crystallography was prepared.

铜胺氧化酶（CAO）催化各种脂肪胺氧化成相应的醛、氨和过氧化氢。我专注于结构的洞察到CAO的催化机制，从节杆菌球形（AGAO）使用超高分辨率的X射线晶体学和中子晶体学方法。对于超高分辨率X射线晶体学，在无衬底形式下以1.08 A分辨率确定AGAO的晶体结构。基于结构，氧的活性中心的途径进行了估计。另一方面，在AGAO与不可逆抑制剂复合的结构的基础上，获得了底物特异性的结构见解。此外，制备了用于中子晶体学的AGAO大晶体。

项目成果

銅アミン酸化酵素のTPQ生成反応と触媒反応における保存性Asn残基の役割

保守的 Asn 残基在 TPQ 生产和铜胺氧化酶催化中的作用

• 发表时间: 2014
• 作者: Takeshi Murakawa;Hideyuki Hayashi;Katsuyuki Tanizawa;Toshihide Okajima;村川武志，中西将太，元山暁仁，林秀行，谷澤克行，岡島俊英
• 通讯作者: 村川武志，中西将太，元山暁仁，林秀行，谷澤克行，岡島俊英

High-resolution crystal structure of copper amine oxidase from Arthrobacter globiformis: assignment of bound diatomic molecules as O2

球状节杆菌铜胺氧化酶的高分辨率晶体结构：将结合的双原子分子指定为 O2

• DOI: 10.1107/s0907444913023196
• 发表时间: 2013
• 期刊: Acta Crystallographica Section D
• 作者: Murakawa T;Hayashi H;Sunami T;Kurihara K;Tamada T;Kuroki R;Suzuki M;Tanizawa K;Okajima T.
• 通讯作者: Okajima T.

Acid-base chemistry of the reductive half reaction of bacterial copper amine oxidase

细菌铜胺氧化酶还原半反应的酸碱化学

• 发表时间: 2011
• 作者: Takeshi Murakawa;Toshihide Okajima;Akio Hamaguchi;Katsuyuki Tanizawa;and Hideyuki Hayashi
• 通讯作者: and Hideyuki Hayashi

Structural insights into the substrate specificity of bacterial copper amine oxidase obtained by using irreversible inhibitors

使用不可逆抑制剂获得的细菌铜胺氧化酶底物特异性的结构见解

• 发表时间: 2012
• 期刊: J. Biochem.
• 作者: Murakawa T;Hayashi H;Taki M;Yamamoto Y;Kawano Y;Tanizawa K;Okajima T.
• 通讯作者: Okajima T.

Structural insights into the substrate specificity of bacterial copper amine oxidase obtained by using irreversible inhibitors.

使用不可逆抑制剂获得的细菌铜胺氧化酶底物特异性的结构见解。

• 发表时间: 2012
• 期刊: THE JOURNAL OF BIOCHEMISTRY
• 作者: Takeshi Murakawa;Hideyuki Hayashi;Masayasu Taki,Yukio Yamamoto;Yoshiaki Kawano;Katsuyuki Tanizawa;Toshihide Okajima.
• 通讯作者: Toshihide Okajima.