Tracking of the bacterial flagellar rotation using single fluorescent microscopy

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The bacterial flagellar motor is a reversible rotary nano-machine, which is powered by ion flux across the cell membrane. The motor has several interesting features as fast rotation, regulation system of torque generation and so on. Their rotation, however, have been measured via flagellar filaments extend from cell body, rather than via the motor basal body directly. To track its rotation directly at molecular level, we have developed single molecule fluorescent microscopy and methods to label the motor basal body.