Development of accurate electrostatic potential imaging for visualization of protein function

在本研究中，为了精确计算电子密度，我尝试开发低分辨率衍射数据的精确测量方法和溶剂面积结构的无误差可视化方法。我在SPring-8上构建了一个高通量x射线精确低分辨率数据测量系统。该系统可获得低分辨率区域的无损耗衍射数据。重原子PbEDTA或Xe可以成功地浸泡在蛋白质晶体中，以显示溶剂面积。

In this research, in order to calculate precise electron density, I tried to develop accurate measurement methods of low resolution diffraction data and visualization methods of solvent area structure without error. I constructed a measurement system of precise low resolution data at SPring-8 with high flux X-ray. Los-free diffraction data in low resolution area could be obtained in this system. Heavy atoms, PbEDTA or Xe, for visualization of solvent area could successfully soaked in protein crystal.