Genetic regulation of bolting time in lettuce

生菜抽苔时间的遗传调控

• 批准号: 23780023
• 负责人: LEE ONEW
• 金额: $ 2.75万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23780023/
• 关键词: レタス; 抽だい; QTL解析; RAD シーケンス; 連鎖地図; RADマーカー; 抽台

Lettuce (Lactuca sativa) plants elongate their flower stalks (bolting) when grown under high temperature and long day-length. Here we conducted QTL analysis to know the genetic control of bolting time in lettuce. We used a F5 population developed from two lettuce cultivars Chimasanchu (early bolting type) and Redfire (late bolting type) to measure days to bolting, flowering and seed formation. We developed SNPs markers using RAD-tag sequence and constructed a linkage map. Composite interval mapping detected 2 QTLs for bolting on linkage group 1 and 16. The presence of Redfire in the detected QTL on linkage group 16 accounted for 58% of phenotypic variation. Further study is needed to increase number of markers on linkage map to perform more precise QTL analysis.

生菜(Lactuca Sativa)植物在高温和长日照条件下生长时，其花梗(抽苔期)会延长。在此，我们进行了QTL分析，以了解生菜抽苔期的遗传控制。我们利用由两个生菜品种Chimasancu(早抽苔型)和Redfire(晚抽苔型)发展而成的F5群体来测量抽蕾、开花和种子形成的天数。我们利用RAD-Tag序列开发了SNPs标记，构建了连锁图谱。复合区间定位在连锁群1和16上检测到2个抽苔QTL，其中在连锁群16上检测到的红火QTL可解释58%的表型变异。为了进行更精确的QTL分析，还需要进一步研究增加连锁图谱上的标记数量。