Genetic regulation of bolting time in lettuce

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Lettuce (Lactuca sativa) plants elongate their flower stalks (bolting) when grown under high temperature and long day-length. Here we conducted QTL analysis to know the genetic control of bolting time in lettuce. We used a F5 population developed from two lettuce cultivars Chimasanchu (early bolting type) and Redfire (late bolting type) to measure days to bolting, flowering and seed formation. We developed SNPs markers using RAD-tag sequence and constructed a linkage map. Composite interval mapping detected 2 QTLs for bolting on linkage group 1 and 16. The presence of Redfire in the detected QTL on linkage group 16 accounted for 58% of phenotypic variation. Further study is needed to increase number of markers on linkage map to perform more precise QTL analysis.