Characterization of the reversion mechanisms of polyphosphate-accumulating mutants

我们之前发现，细菌磷酸盐调控子的负调节因子phou的突变会导致细胞中多磷酸盐（polyP）水平升高。然而，PhoU突变体迅速超越了失去积累息肉能力的逆转体。在本研究中，我们分析了返归体丧失聚酰亚胺积累能力的机制，并建立了一种筛选稳定PhoU突变体的方法。本研究的发现将适用于磷回收技术的开发。

We previously revealed that a mutation inPhoU, a negative regulator of bacterial phosphate regulon, results in the elevated levels of polyphosphate (polyP) in cell. However, PhoU mutants were rapidly outgrownby revertant(s) that lost the ability to accumulate polyP. In this study, we analyzed the mechanisms that the revertants lost polyP-accumulating ability, and established a method to screen stable PhoU mutants. The discovery in this study would be applicable to the development of a phosphorus-recovering technology.