Characterization of the reversion mechanisms of polyphosphate-accumulating mutants

我们先前揭示了细菌磷酸盐调节子的负调节因子PhoU的突变导致细胞中多磷酸盐（polyP）水平升高。然而，PhoU突变体很快就被失去积累polyP能力的回复突变体所淘汰。本研究分析了PhoU突变体丧失聚磷积累能力的机理，建立了一种筛选稳定PhoU突变体的方法。本研究的发现将适用于磷回收技术的开发。

We previously revealed that a mutation inPhoU, a negative regulator of bacterial phosphate regulon, results in the elevated levels of polyphosphate (polyP) in cell. However, PhoU mutants were rapidly outgrownby revertant(s) that lost the ability to accumulate polyP. In this study, we analyzed the mechanisms that the revertants lost polyP-accumulating ability, and established a method to screen stable PhoU mutants. The discovery in this study would be applicable to the development of a phosphorus-recovering technology.