Microbial ecology of nitrous oxide-reducing denitrifying bacteria in the livestock waste composts

畜禽粪便堆肥中一氧化二氮还原反硝化细菌的微生物生态学

• 批准号: 23780087
• 负责人: OHTSUBO Wakako
• 金额: $ 2.5万
• 依托单位: Tohoku Gakuin University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23780087/
• 关键词: 亜酸化窒素; 堆肥化プロセス; 脱窒; 群集構造解析; アンモニア酸化; 細菌群集構造解析

In this study we performed composting experiments using solidwastes from piggery wastewater treatment processes. Our results indicated that the timing of N_2O emission follows emergence of ammonia-oxidizing bacteria, which produce denitrification substrates such as nitrate and nitrite. We also found that hyperthermophilic pre-treatment reduces N_2O emission from composting, which is suggest to have been caused by denitrifying bacteria with N_2O-reducing activities. Phylogenetic analyses showed that community structures of ammonia-oxidizing bacteria and denitrifying bacteria dynamically changed as composting proceeded. Denitrifying bacterial strains isolated from our compost samples were able to perform denitrification under oxic or microoxic conditions. These findings collectively suggest that N_2O emission patterns during composting is strongly affected by community structures and functions of ammonia-oxidizing bacteria and denitrifying bacteria, which should be effectively controlled to reduce N_2O emission from composting processes.

在这项研究中，我们利用养猪场废水处理过程中的固体废物进行了堆肥试验。我们的结果表明，N2O的释放时间与氨氧化细菌的出现有关，氨氧化细菌会产生硝酸盐和亚硝酸盐等反硝化底物。我们还发现，高温预处理减少了堆肥产生的N2O，这可能是由具有N2O还原活性的反硝化细菌引起的。系统发育分析表明，氨氧化细菌和反硝化细菌的群落结构随着堆肥的进行而发生动态变化。从堆肥样品中分离到的反硝化细菌能够在好氧或微氧条件下进行反硝化。这些结果表明，堆肥过程中N2O的排放模式受到氨氧化细菌和反硝化细菌群落结构和功能的强烈影响，应对其进行有效控制，以减少堆肥过程中的N2O排放。

项目成果

堆肥化プロセスにおける亜酸化窒素(N2O) 発生に硝化・脱窒細菌が与える影響

硝化和反硝化细菌对堆肥过程中一氧化二氮（N2O）生成的影响

• 发表时间: 2013
• 作者: 大坪和香子;進藤絵里香;宮内啓介;遠藤銀朗;東海林晃;大坪和香子
• 通讯作者: 大坪和香子

Nitrous oxide (N_2O) emission during composting: when and how?

堆肥过程中一氧化二氮 (N_2O) 的排放：何时以及如何排放？

• 发表时间: 2012
• 作者: 大坪和香子;尾形裕明;宮内啓介;遠藤銀朗;大坪和香子
• 通讯作者: 大坪和香子

Effect of mismatches in primers for amplifying nitrous oxide reductase (nosZ) genes on communitystructure analysis of denitrifying bacteria

一氧化二氮还原酶（nosZ）基因引物错配对反硝化细菌群落结构分析的影响

• 发表时间: 2012
• 作者: Wakako Ikeda-Ohtsubo;Erika Shindo;Keisuke Miyauchi;Ginro Endo
• 通讯作者: Ginro Endo

Nitrous oxide (N2O) emission during composting – when and how?

堆肥过程中一氧化二氮 (N2O) 的排放——何时以及如何排放？

• 发表时间: 2012
• 作者: Shu Minagawa;Hiroyuki Inami;Tomohisa Kato;Shinji Sawada;Tatsuya Yasuki;Shinichi Miyairi;Manabu Horikawa;Jun Okuda;Naomasa Gotoh.;Wakako Ikeda-Ohtsubo
• 通讯作者: Wakako Ikeda-Ohtsubo

Community structure and population dynamics of ammonia oxidizers in composting processes of ammonia-rich livestock waste.

• DOI: 10.1016/j.syapm.2013.02.001
• 发表时间: 2013-07
• 期刊: Systematic and applied microbiology
• 影响因子: 3.4
• 作者: T. Yamada;Shinya Araki;W. Ikeda-Ohtsubo;K. Okamura;A. Hiraishi;Hideyo Ueda;Yasuichi Ueda;K. Miyauchi