Elucidation of production mechanism of peptidyl-tRNAs dropped from ribosomes in bacterial translation system

阐明细菌翻译系统中从核糖体上脱落的肽基-tRNA 的产生机制

• 批准号: 23780102
• 负责人: NAGAO Asuteka
• 金额: $ 2.91万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23780102/
• 关键词: 遺伝子発現; 翻訳; 翻訳精度維持機構; tRNA; リボソーム; rRNA

We directly analyzed and characterized peptidyl-tRNAs which were dissociated from the ribosome during elongation by using LC/MS analyses and RNA isolation technique. When each individual pep-tRNA was isolated and analyzed in detail, we found that noncognate peptidyl-tRNAs whose nascent chains didn’t match any of E.coli genes were also dropped from the ribosome. This suggested a novel quality control mechanism after peptidyltransfer reaction, namely noncognate peptidyl-tRNAs can berejected from the ribosome.

我们利用LC/MS分析和RNA分离技术直接分析和表征了在延伸过程中从核糖体分离的肽基trna。当每个单独的肽- trna被分离出来并进行详细分析时，我们发现新生链与大肠杆菌基因不匹配的非同源肽- trna也从核糖体中脱落。这提示了一种新的肽基转移反应后的质量控制机制，即非同源肽基trna可以从核糖体中分离出来。

项目成果

翻訳初期段階におけるペプチジルtRNAの脱落と校正機構

翻译早期阶段的肽基-tRNA脱落和校对机制

• 发表时间: 2016
• 作者: Hiroki Takai;Tetsu Akiyama et al.;Tsutomu Suzuki and Tomoyuki Numata;Tsutomu Suzuki;Tsutomu Suzuki;鈴木 勉;Tsutomu Suzuki;鈴木 勉;Tsutomu Suzuki;Tsutomu Suzuki;Tsutomu Suzuki;鈴木 勉;Tsutomu Suzuki;鈴木 勉;鈴木 勉;Tsutomu Suzuki;Tsutomu Suzuki;Tsutomu Suzuki;鈴木 勉
• 通讯作者: 鈴木 勉