Elucidation of novel enzymatic system involved in the activation of the defensive secondary metabolites in the tulip

阐明参与郁金香防御性次生代谢物激活的新型酶系统

• 批准号: 23780120
• 负责人: NOMURA Taiji
• 金额: $ 2.91万
• 依托单位: Toyama Prefectural University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23780120/
• 关键词: チューリップ; チューリッポシド; チューリッパリン; チューリッポシド変換酵素; カルボキシルエステラーゼ; 二次代謝; 生合成; 酵素

This study was performed to identify the enzymes involved in the conversion of tuliposides (Pos), the major secondary metabolites in tulip, to antimicrobial tulipalins (Pa), the lactonized aglycons of Pos. I found that PosA and PosB, which are the major forms of Pos in the tulip cultivar, are converted to the corresponding PaA and PaB by PosA-converting enzyme and PosB-converting enzyme, respectively. Amino acid sequences of those enzymes were similar to the carboxylesterases, which typically catalyze hydrolysis reactions. However, the Pos-converting enzymes catalyzed no hydrolysis of Pos, but the formation of Pa through lactonization. Pos-converting enzymes were identified as the unique lactone-forming carboxylesterases, specifically catalyzing intramolecular transesterification.

本研究旨在确定郁金香中的主要次生代谢物郁金香糖苷(POS)转化为抗菌郁金香蛋白(PA)所涉及的酶。我发现，郁金香品种中POS的主要形式POSA和PosB分别被POSA转换酶和PosB转换酶转化为相应的PAA和PAB。这些酶的氨基酸序列与羧酸酯酶相似，后者通常催化水解反应。然而，POS转换酶并不催化POS的水解，而是通过内酯作用形成PA。POS转化酶被鉴定为唯一的内酯形成羧酸酯酶，专门催化分子内酯交换反应。

项目成果

A novel lactone-forming carboxylesterase from tulip

一种来自郁金香的新型内酯形成羧酸酯酶

• 发表时间: 2012
• 作者: Yasuo Kato;Shinjiro Ogita;Taiji Nomura
• 通讯作者: Taiji Nomura

Molecular characterization of tuliposide A-converting enzyme in tulip

郁金香郁金香苷 A 转化酶的分子表征

• 发表时间: 2011
• 作者: Taiji Nomura;Shinjiro Ogita;Yasuo Kato
• 通讯作者: Yasuo Kato

Molecular Diversity of Tuliposide A-Converting Enzyme in the Tulip

郁金香中郁金香苷A转换酶的分子多样性

• DOI: 10.1271/bbb.130021
• 发表时间: 2013
• 期刊: Bioscience, Biotechnology, and Biochemistry
• 作者: Nomura;T.;Tsuchigami;A.;Ogita;S. &Kato;Y.
• 通讯作者: Y.

Occurrence of hydroxycinnamoylputrescines in xylogenic bamboo suspension cells

木源竹悬浮细胞中羟基肉桂酰腐胺的存在

• DOI: 10.5511/plantbiotechnology.13.0704a
• 发表时间: 2013
• 期刊: Plant Biotechnology
• 影响因子: 1.6
• 作者: Nomura;T.;Shiozawa;M.;Ogita;S. & Kato;Y.
• 通讯作者: Y.

Molecular diversity of tuliposide A-converting enzyme in tulip

郁金香郁金香苷A转化酶的分子多样性

• 发表时间: 2012
• 作者: Taiji Nomura;Shinjiro Ogita;Yasuo Kato
• 通讯作者: Yasuo Kato