A study on search of protein species which contribute to growth of a harmful alga Chattonella

寻找有助于有害藻类查托氏菌生长的蛋白质种类的研究

• 批准号: 23780197
• 负责人: SHIMASAKI Yohei
• 金额: $ 2.91万
• 依托单位: Kyushu University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23780197/
• 关键词: シャトネラ; プロテオミクス; 2-Ｃys Ｐｒｘ; 過酸化水素; 増殖能力; 赤潮; 光合成活性; peroxidoredoxin

Present study aimed at identifying molecular markers reflecting physiological status response to Chattonella marina growth and investigating temporal variation in the potential maximum quantum yield of photosystem II (Fv/Fm ratio) via a field survey and laboratory cultures. Proteome profiles obtained from 2-DE suggested that 2-cysteine peroxiredoxin (2-Cys Prx) play a role in maintaining positive growth of C. marina. The Fv/Fm ratio gradually decreased from approximately 0.72 to 0.62, and the growth rate of C. marina also decreased from approximately 0.5 to <0 div/day during the bloom; there was a significant positive correlation between the two parameters.Our results suggest that the expression level of 2-Cys Prx and Fv/Fm ratio, combined with information about environmental factors, may be useful in evaluating the growth potential of C. marina during field blooms.

本研究旨在通过田间调查和室内培养，寻找反映轮藻生长生理状态的分子标记，并研究光系统II潜在最大量子产率(Fv/Fm)随时间的变化。双向凝胶电泳法得到的蛋白质组图谱表明，2-半胱氨酸过氧化还蛋白(2-Cys PRX)在维持滨海梭子蟹正向生长中起着重要作用。结果表明，2-Cys Prx和Fv/Fm比值的表达水平和Fv/Fm比值，结合环境因子的信息，可以用来评价大田水华的生长潜力。

项目成果

有害ラフィド藻Chattonellaの光合成活性と増殖との関係

有害藻类查托氏菌光合活性与增殖的关系

• 发表时间: 2013
• 作者: Abkallo HM;Kawano H;Watanabe K,Kobayashi N;Xuchun Qiu・島崎洋平・松原賢・本城凡夫・大嶋雄治;Qiu Xuchun ・島崎 洋平 ・松原 賢 ・本城 凡夫 ・大嶋 雄治;島崎洋平・本田清一郎・吉田光男・本城凡夫・望岡典隆・沖野望・Xuxhun Qiu・大嶋雄治;寺本彩乃・島崎洋平・溝口直洋・Qiu Xuchun・鵜木陽子・津山孝人・中島悠・本城凡夫・大嶋雄治;Qiu Xuchun・島崎洋平・津山孝人・本田匡人・溝口直洋・松原賢・本城凡夫・大嶋雄治
• 通讯作者: Qiu Xuchun・島崎洋平・津山孝人・本田匡人・溝口直洋・松原賢・本城凡夫・大嶋雄治

Allelopathy of the raphidophyte Heterosigma akashiwo against the dinoflagellate Akashiwo sanguinea is mediated via allelochemicals and cell contact

• DOI: 10.3354/meps09476
• 发表时间: 2012-02
• 期刊: Marine Ecology Progress Series
• 影响因子: 2.5
• 作者: Xuchun Qiu;Y. Yamasaki;Y. Shimasaki;Hiroaki Gunjikake;Masato Honda;Mayumi Kawaguchi;T. Matsubara;S. Nagasoe;T. Etoh;S. Matsui;T. Honjo;Y. Oshima

ラフィド藻Chattonella marina細胞中の過酸化水素消去酵素peroxidoredoxinの遺伝子解析

粘杆藻海藻细胞中过氧化氢清除酶过氧化还原酶的遗传分析

• 发表时间: 2013
• 作者: Abkallo HM;Kawano H;Watanabe K,Kobayashi N;Xuchun Qiu・島崎洋平・松原賢・本城凡夫・大嶋雄治;Qiu Xuchun ・島崎 洋平 ・松原 賢 ・本城 凡夫 ・大嶋 雄治;島崎洋平・本田清一郎・吉田光男・本城凡夫・望岡典隆・沖野望・Xuxhun Qiu・大嶋雄治;寺本彩乃・島崎洋平・溝口直洋・Qiu Xuchun・鵜木陽子・津山孝人・中島悠・本城凡夫・大嶋雄治
• 通讯作者: 寺本彩乃・島崎洋平・溝口直洋・Qiu Xuchun・鵜木陽子・津山孝人・中島悠・本城凡夫・大嶋雄治

Temporal variations in photosynthetic activity and phase-dependent cellular protein expression during growth of harmful raphidophyte Chattonella antiqua

有害针叶植物古查托氏菌生长过程中光合活性和相位依赖性细胞蛋白表达的时间变化

• 发表时间: 2012
• 作者: Qiu X;Shimasaki Y;Tsuyama M;Yamada T;Honda M;Kato-Unoki Y;Nakashima T;Yamasaki Y;Honjo T;Oshima Y
• 通讯作者: Oshima Y

Maximum Quantum Yield of Photosystem II and Photosynthesis Related Protein Expression Levels as Possible Bio-indicators of Growth Potential in Harmful Raphidophyte Chattonella antiqua

光系统 II 的最大量子产率和光合作用相关蛋白表达水平作为有害剑藻古查托氏菌生长潜力的可能生物指标

• 发表时间: 2011
• 作者: Qiu X;Shimasaki Y;Tsuyama M;Shimizu M;Yamada T;Kuwahara R;Kawaguchi M;Sato Y;Kato-Unoki Y;Nakajima T;Matsubara T;Yamasaki Y;Ichinose H;Wariishi H;Honjo T;Oshima Y
• 通讯作者: Oshima Y