The elucidation of the apoptotic mechanism in cryopreserved bovine oocytes and the establishment of the new method for cryopreservation and in vitro culture

为了进一步完善牛卵母细胞（萌发泡期）的冷冻保存方法，本研究分析了冷冻保存对卵母细胞的影响。冻存改变了线粒体和微管的定位，导致线粒体功能下降，诱导细胞凋亡。然后，我们成功地提高了成熟，受精和发育率的冷冻保存的卵母细胞的细胞骨架稳定剂的处理和卵丘细胞的去除。此外，为了避免受精障碍的冷冻保存的卵母细胞，我们分析了控制精子的生育力的因素。发现精子运动的直线性、运动速度和顶体正常性对精子的生育力有影响。

For the purpose of the improvement of the cryopreservation method in bovine oocytes (germinal vesicle stage), we analyzed the effect of the cryopreservation on oocytes. The cryopreservation changed mitochondrial and microtublar localization, as a result, mitochondrial functional decline and induction of the apoptosis occurred. Then, we were successful in increasing the rates of maturation, fertilization and development in the cryopreserved oocytes by the treatment of the cytoskeleton stabilizer and removal of the cumulus cells.Also, for the purpose of evading a fertilization disorder of the cryopreserved oocytes, we analyzed of the factor which controlled fertility of the sperm. We found that linearity and velocity of moving, and acrosomal normality had influence to fertility of the sperm.