Is mitochondrial DNA repair mediated by AP endonuclease involved in the pathophysiology of cancer?

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Mitochondrial AP endonuclease (mtAPE), which is unidentified at present, is assumed to be important for mitochondrial DNA repair. This study was aimed to elucidate the identity and function of mtAPE. In this study, we examined the subcellular localization of AP endonuclease 1, which is one of mtAPE candidates, and observed no APE1 distribution in pure mitochondrial fraction. The enzymatic activity tests revealed that there was some AP endonuclease activity and substantial AP lyase activity in pure mitochondrial fraction. Furthermore, we also found that the molecular weight of this AP lyase was between 30 and 40 kD.