Analysis of interaction between novel prion protein (PrP) family Shadoo and PrP

为探讨朊蛋白（PrP）表达对影子蛋白（Sho）表达的影响，分析了PrP缺陷细胞系（HpL）血清剥夺前后Sho表达水平的变化。在血清存在的情况下，观察到HpL和表达PrP的HpL之间Sho的表达水平相似，表明PrP的表达不影响Sho的水平。此外，血清剥夺并未改变HpL和HpL- prp细胞中Sho的表达水平。提示PrP的抗凋亡功能与Sho的表达无关。

To investigate the influence of prion protein (PrP) expression on Shadoo (Sho) expression, the levels of Sho in PrP-deficient cell line (HpL) was analyzed before and after serum deprivation. In the presence of serum, the similar expression levels of Sho between HpL and HpL expressing PrP were observed, suggesting that the PrP expression did not affect the levels of Sho. In addition, serum deprivation did not change the expression levels of Sho in both HpL and HpL-PrP cells. These suggest anti-apoptotic function of PrP is independent of Sho expression.