Association between siderophores produced by pathogenic vibrios and host inflammatory response

致病性弧菌产生的铁载体与宿主炎症反应之间的关联

• 批准号: 23790166
• 负责人: TANABE Tomotaka
• 金额: $ 2.75万
• 依托单位: Matsuyama University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23790166/
• 关键词: シデロフォア; 鉄; 病原ビブリオ; 腸炎ビブリオ; ビブリオ属菌

Iron is an essential element for bacterial survival, and its acquisition is crucial for the ability of most pathogenic bacteria to establish and maintain an infection within the host. Vibrio parahaemolyticus, a human pathogen causing watery diarrhea, can produce vibrioferrin, a type of siderophore which is a low-molecular-weight iron-binding chelator produced by microbes, and can acquire iron provided by iron-vibrioferrin complex. In this study, I clarified a novel regulatory system for production of vibrioferrin. Moreover, I examined a physiological function of vibrioferrin against host cells, and also assessed the influence of the deletion of gene involved in vibrioferrin production on cytotoxicity of V. parahaemolyticus.

铁是细菌生存的必需元素，它的获得对于大多数病原菌在宿主内建立和维持感染的能力至关重要。副溶血性弧菌（Vibrio parahaemolyticus）是一种引起人类水样腹泻的病原菌，它能产生铁载体（Vibrioferrin），铁载体是由微生物产生的一种低分子量的铁结合螯合剂，并能从铁-铁载体复合物中获得铁。在这项研究中，我澄清了一个新的生产vibrioferrin的调节系统。此外，我检查了Vibrioferrin对宿主细胞的生理功能，并评估了参与Vibrioferrin生产的基因缺失对副溶血性弧菌细胞毒性的影响。

项目成果

The Vibrio parahaemolyticus small RNA ryhB promotes production of the siderophore vibrioferrin by stabilizing the polycistronic mRNA.

副溶血弧菌小 RNA ryhB 通过稳定多顺反子 mRNA 来促进铁载体弧菌铁蛋白的产生。

• DOI: 10.1128/jb.00162-13
• 发表时间: 2013
• 期刊: J. Bacteriol.
• 作者: Tanabe;T.;Funahashi;T.;Nakao;H.;Maki;J.;Yamamoto;S.
• 通讯作者: S.

The Vibrio parahaemolyticus psuA gene encodes a second ferric vibrioferrin receptor exclusively dependent on the TonB2 system.nternational

副溶血性弧菌 psuA 基因编码第二种铁弧菌铁蛋白受体，完全依赖于 TonB2 系统。

• 发表时间: 2011
• 作者: Tanabe;T.;Funahashi;T.;Nakao;H.;Miyamoto;K.;Tsujibo;H.;and Yamamoto;S.
• 通讯作者: S.

Utilization of xenosiderophores by Vibrio parahaemolyticus:identification and characterization of genes, irgA, vctA and vpa0150, encoding ferric enterobactin receptors.

副溶血弧菌对异铁载体的利用：编码铁肠杆菌素受体的基因 irgA、vctA 和 vpa0150 的鉴定和表征。

• 发表时间: 2011
• 作者: 宮本勝城; 辻坊 裕
• 通讯作者: 辻坊 裕

Utilization of xenosiderophores by Vibrio parahaemolyticus: Idenfitication and characterization of genes, irgA, vctA and VPA0150, encoding ferric enterobactin receptors

副溶血弧菌对异铁载体的利用：编码肠杆菌素铁受体的基因 irgA、vctA 和 VPA0150 的鉴定和表征

• 发表时间: 2011
• 作者: Tomotaka Tanabe;Tatsuya Funahashi;Katsushiro Miyamoto;Hiroshi Tsujibo;Shigeo Yamamoto
• 通讯作者: Shigeo Yamamoto

The Vibrio parahaemolyticus pvuA1 gene (formerly termed psuA) encodes a second ferric vibrioferrin receptor that requires tonB2.

• DOI: 10.1111/j.1574-6968.2011.02389.x
• 发表时间: 2011-11
• 期刊: FEMS microbiology letters
• 影响因子: 2.1
• 作者: T. Tanabe;Tatsuya Funahashi;Noriyuki Okajima;H. Nakao;Y. Takeuchi;K. Miyamoto;H. Tsujibo;Shigeo Yamamoto