The elucidation of the humoral immunity control mechanism by heme

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The transcriptional repressor Bach2 possesses the basic region-leucine zipper (bZip) motif that mediates dimer formation with Maf oncoproteins and DNA binding. It has been found that heme inhibits the DNA binding activity of Bach2 in vitro.and induces degradation of Bach2 in B cells. To reveal the molecular mechanism of the heme-mediated regulation of Bach2, we mapped the heme-binding region by using spectroscopic analyses. Moreover, the results of various instrumental analysis of Bach2 revealed that the unstructured region of Bach2 is important for heme binding and/or transducing heme effect upon the functional regulation of Bach2.