An investigation into functional roles of microRNA inthe animal model of malignant peritoneal mesothelioma

microRNA （miRNA）微阵列的初步实验揭示了miRNA-199/214是糖铁诱导的肉瘤样恶性间皮瘤的标志。在这项资助的帮助下，通过实时PCR和ISH证实miRNA-199/214的升高。Twist-1转录miRNA-199/214，在骶样间皮瘤和石棉诱导的恶性腹膜间皮瘤及培养细胞系中检测到。将miR-199/214转染到永生化间皮细胞系中可以增加细胞生长，这为miRNA-199/214的功能作用提供了证据。

Preliminary experiment of microRNA (miRNA) microarray disclosed miRNA-199/214 as a hallmark of iron saccharate-induced sarcomatoid malignant mesothelioma. With the aid of this grant, elevation of miRNA-199/214 was confirmed by real-time PCR and ISH. Twist-1, which transcribes miRNA-199/214, was detected in sacromatoid mesothelioma and asbestos-induced malignant peritoneal mesothelioma and cultured cell lines. Transfection of miR-199/214 into immortalized mesothelial cell line increased cell growth, providing evidence of a functional role of miRNA-199/214.