Analysis of the factors of host-parasite relationship in severe malaria pathology

1)为了确认SR的配体，在PlasmoDB上搜索候选蛋白。鉴定了其中4种疟原虫蛋白，并利用E. coli，并确认其生物学功能。2)在感染P. B e rg h e i ANKA（PbANKA）的小鼠中观察到SRs的表达。在肺、肝和脾中，PbANKA感染显著增强了A类SR MARCO的表达，但未增强B类SR CD 36的表达。MARCO在边缘区巨噬细胞和肝窦内皮细胞上均有表达。应用基因芯片技术检测Py 17 XL感染致死组和恢复组小鼠基因表达的差异。与致死组相比，恢复组59304基因中的5893基因表达增强，提示致死组（M2a巨噬细胞）和恢复组（M2c样巨噬细胞）均诱导出不同类型的巨噬细胞。

1) To confirm the ligands to SRs, the candidate proteins were searched on the PlasmoDB. Four of the Plasmodiumproteins were identified and their recombinant proteins were successfully made by common methods using E. coli, and now confirming their biological functions. 2) The expression of SRs was observed in mice infected with P. b e r g h e i ANKA (PbANKA). The expression of class A SR, MARCO, was markedly enhanced by PbANKA infection in lung, liver, and spleen, but not class B SR, CD36. MARCO was histopathologically expressed on the macrophages in marginal zone macrophages and endothelial cells in liver sinusoid. The differentiation of gene expression in both lethal and recovered mice group from P. y o e l l i17XL (Py17XL) infection were measuredby DNA microarray. The expression of 5893 in 59304 genes was enhanced in recovered group compared with lethal it. The results suggested that different types of macrophages were induced in both lethal (M2a macrophage) and recovered mice (M2c-like macrophage).