Establishment of novel HCV cell-culture system using HCV strains other than JFH-1

我们利用含有NS4A、NS5B和3‘UTR5个突变的J6CF株建立了一个新的丙型肝炎病毒2a型细胞培养系统。我们还利用含有JFH-1最小区域的MA株嵌合基因组建立了一个丙型肝炎病毒2b基因的细胞培养系统。这些细胞培养系统将有助于确定2a和2b基因型病毒的特征，并开发抗病毒策略。我们的数据提供的信息将被证明对建立目前在培养细胞中不具有复制能力的丙型肝炎病毒株的复制能力变体至关重要。

We established a novel HCV genotype 2a cell-culture system using J6CF strain harboring five mutations in NS4A, NS5B and 3'UTR. We also established an HCV genotype 2b cell-culture system using a chimeric genome of MA strain harboring minimal regions of JFH-1. These cell-culture systems will be useful for characterizing genotype 2a and 2b viruses and developing antiviral strategies. Our data provides information that will prove essential for the establishment of replication-competent variants of HCV strains that are currently replication incompetent in cultured cells.