Imaging of mixed body fluid stain by MALDI-TOF-MS

MALDI-TOF-MS 混合体液染色成像

• 批准号: 23790734
• 负责人: FUJINAMI Yoshihito
• 金额: $ 1.66万
• 依托单位: National Research Institute of Police Science
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23790734/
• 关键词: MALDI-TOF-MS; イメージング; 精液; 体液斑; 質量分析; MALDI; mass imaging; マススペクトル; 膣液

It was detected as characteristic macromolecule at high intensity on m/z 2890 in semen and seminal stain extract from ten healthy young volunteers. In addition, a sheet of cotton cloth attaching each stain of semen, blood, urine, and saliva was transferred to a sheet of conductive double sided carbon adhesive tape, following that the locations of seminal stains were detected by MALDI-imaging with m/z 2890 (as a molecular marker) on the replica. MALDI-imaging might be a useful tool for screening of the locations of seminal stains before tests that have been used and are currently trusted

在10名健康青年志愿者的精液和精斑提取物中，在m/z 2890处检测到高强度的特征性大分子。此外，将附着精液、血液、尿液和唾液的每种污渍的棉布片转移到导电双面碳胶带片上，然后通过在复制品上使用m/z 2890（作为分子标记）的MALDI成像检测精液污渍的位置。MALDI成像可能是一个有用的工具，用于在测试之前筛选精斑的位置，这些测试已经使用并且目前是可信的