Search for infectious agents in pancreatitis by next-generation sequencing

绝大多数胰腺炎发作的起源是非传染性的，感染因子在这种疾病中的作用仍然存在争议。新一代测序技术在病毒学领域有许多潜在的应用，如发现新病毒、检测临床标本中意想不到的病毒病原体等。在本研究中，我们没有发现新的病毒作为胰腺炎的起源。急性胰腺炎死亡的主要原因是胰腺组织继发感染。甘露糖结合凝集素（MBL）是机体免疫的主要成分之一。我们证明，与没有感染并发症的患者相比，有感染并发症的患者MBL基因密码子54突变的患病率略高（50%对27%）。

The vast majority of episodes of pancreatitis are noninfectious in origin, and the role of infectious agents in this disorder remains controversial. Next-generation sequencing has many potential applications in virology, such as discovery of new viruses, and detection of unexpected viral pathogens in clinical specimens. In this study, we could not discover of new viruses as the origin of pancreatitis. The major cause of death by acute pancreatitis is secondary infection of pancreatic tissue. Mannose-binding lectin (MBL) is one of the main components of immunity. We demonstrated that the prevalence of the codon 54 mutation of MBL gene in patients with infectious complications was slightly higher compared with patients without infectious complications (50% vs 27%).