The possibility of unknown viral infection with primary biliary cirrhosis and the disease association with anellovirus infection.

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We tried to sequence hepatitis C virus (HCV) genome with RDV method, before conducting RDV method with serum in PBC patients or NOD. c3c4 mice. But this method seemed to be impossible to detect unknown viral sequence. Therefore, we tried to use deep sequencing technology.Result: The reads of HCV origin mapped 8583 reads on the reference HCV genome. The reads of HCV origin were detectable in about 0.10% of the total reads. Surprisingly, the coverage showed 98.8% and read depth indicated 69.5x. In NS3, the position of 5 amino acid bases showed mixed variants.