Role of naofen, a novel WD 40-repeat protein, in diabetic angiopathy

高糖可诱导细胞产生活性氧(ROS)并诱导细胞凋亡，同时伴有p38丝裂原活化蛋白激酶(MAPK)的激活。我们已经报道了一种新的WD40重复蛋白Naofen，它可能通过激活caspase-3来介导细胞凋亡。本研究旨在探讨诺芬在糖尿病血管病变中的潜在作用。我们的结果表明，诺芬在链脲佐菌素诱导的糖尿病大鼠肾脏血管内皮细胞和高糖处理的大鼠动脉内皮细胞中的表达增强。此外，ROS的产生、p38MAPK的激活和NF-κB的激活可能参与了高糖诱导的Naofen表达的增加。

High glucose was shown to elicit reactive oxygen species (ROS) production and induce apoptosis accompanied by activation of p38 mitogen-activated protein kinase (MAPK). We have reported that naofen, a novel WD40-repeat protein, may mediate apoptosis through caspase-3 activation. The present study was undertaken to investigate the potential involvement of naofen in diabetic angiopathy. Our results indicate that enhanced expression of naofen in vascular endothelial cells in kidney of streptozotocin-induced diabetic rats, also in high glucose-treated rat arterial endothelial cells. Furthermore, ROS generation, p38 MAPK activation and NF-κB activation, may be involved in high glucose-induced increase in naofen expression.